刘夫锋，博士，天津科技大学生物工程学院教授，博士生导师。天津市特聘教授、天津市高校学科领军人才。第三届全国发酵工程技术工作委员会专家委员、中国生物发酵产业协会第三届理事会理事、天津市医学影像技术研究会基础医学影像分会常委和香江学者联谊会理事。

主讲课程

本科生：《基因工程》、《生物信息学》和《计算机辅助药物设计与药物信息学》

研究生：《生物信息学与计算生物学》

主要教学研究及成果情况

先后主持或参与教改项目3项，发表教改论文1篇，主编或参编教材或专著2部。

[1] 主持天津市普通高等学校本科教学质量与教学改革研究计划项目，创新创业教育背景下生物工程专业实验课程建设研究与实践，4.0万，2021.10-2023.09。

[2] 主持 2021年天津科技大学研究生教育改革创新类案例库重点项目，生物信息学与计算生物学案例库建设与实践，2.0万，2020.10-2022.09，B201005704。

[3] 主持天津科技大学2018年教育教学改革“创新创业专项”，创新创业教育背景下生物工程专业实验教学内容改革优化研究，0.5万，2017.10-2019.09，2018JGZX003。

[4] 主持天津大学2014年实验教改项目，生物工程专业实验课教学体系的改革与完善，1万，2015.01-2015.12。

[5] 冯远航, 刘夫锋, 朱勇, 白姝, 深化生物工程专业实验教学改革与创新, 实验室科学, 2020, 23(2): 157-160。

[6] Y. Sun*, Q.-H. Shi, L. Zhang, G. Zhao, F. -F. Liu: Adsorption and chromatography. In ed. Comprehensive Biotechnology, Vol. 2, Chapter 2.47. pp. 665-679. Elsevier, 2011.

[7] 贾士儒主编，生物工程专业实验（第二版），中国轻工业出版社，2010。

科研领域及方向

[1] 工业酶分子理性设计

[2] 淀粉样蛋白聚集及其抑制剂开发

[3] 抗老年痴呆功能食品开发

科研项目情况

[1] 国家重点研发计划：“非细胞生物合成系统的构建与应用(2018YFA0901700)（总经费:2460万）”非细胞生物合成途径设计及超稳人工多酶体系组装(2018YFA0901702)，180万，子课题负责人，2019.07-2024.06。

[2] 国家自然科学基金面上项目：Aβ聚集诱导发光体系构建及其在细胞内Aβ聚集抑制剂筛选中的应用，21878234，66万，主持，2019.01-2022.12。

[3] 天津市应用基础及前沿技术研究计划重点项目：聚集诱导荧光探针-α-突触核蛋白融合体系构建及其在聚集抑制剂筛选中的应用，18JCZDJC33000，20万，2018.04-2021.03。

[4] 天津市特聘教授启动经费：天津市教委，100万，2017.07-2022.06。

[5] 天津市高校学科领军人才培养计划：淀粉样蛋白质聚集抑制剂开发， 60万，2017.09-2020.08。

[6] 海河学者特聘教授：蛋白质聚集及其抑制，50万，2016.04-2019.03。

[7] 国家自然科学基金面上项目，细胞膜表面有机小分子抑制淀粉样β多肽构象转换和聚集的多尺度研究，21576199，78.7万，2016.01-2019.12。

[8] 国家自然科学基金青年项目：含羟基类渗透剂稳定蛋白质的分子机理研究，20906068，20万，2010.01-2012.12。

主要学术成果

先后发表各类科研论文100余篇，授权发明专利6项。近5年发表的主要学术论文：

[1] Luying Jiang, Quancheng Sun, Li Li, Fuping Lu, Fufeng Liu*, Molecular insights into inhibitory effect of GV971 components with different sizes against conformational transition of Aβ42 monomer, accepted.

[2] Wenping Zhao#, Luying Jiang#, Wenjuan Wang, Jingcheng Sang, Quancheng Sun, Qinchen Dong, Li Li*, Fuping Lu*, Fufeng Liu*. Design of Carboxylated Single-Walled Carbon Nanotube as High-Efficient Inhibitor against Aβ40 Fibrillation based on HyBER Mechanism, Journal of Materials Chemistry B, 2021, 9 (34): 6902-6914.

[3] Fenghua Wang#, Ying Wang#, Luying Jiang, Wenqian Wang, Jingcheng Sang, Xinyu Wang, Fuping Lu, Fufeng Liu*. The food additive fast green FCF inhibits α‑synuclein aggregation, disassembles mature fibrils and protects against amyloid-induced neurotoxicity. Food & Function. 2021, 12: 5465.

[4] Jialin Li, Luying Jiang; Xue Cao; Yifan Wu; Fuping Lu; Fufeng Liu*; Yu Li*; Yihan Liu*, Improving the activity and stability of Bacillus clausii alkaline protease using directed evolution and molecular dynamics simulation, Enzyme and Microbial Technology, 2021, 147: 109787.

[5] Xiao Guo, Chengcheng Chai, Yajing An, Chong Peng, Nian Shi, Wenqian Wang, Fuping Lu, Yujie Dai*, Fufeng Liu*. Rational design of signal peptides for improved MtC1LPMO production in Bacillus amyloliquefaciens, International Journal of Biological Macromolecules, 2021, 175: 262-269.

[6] 陈贝贝，蒋露莹, 郭芳妍，屈莉莉，汪文倩，金成华*，刘夫锋*. 托卡朋衍生物PCDNA抑制Aβ42纤维化并降低其细胞毒性, 药学学报, 2021, 56(4): 1063-1069.

[7] Fufeng Liu*, Wenjuan Wang, Zhenquan Xuan, Luying Jiang, Beibei Chen, Qinchen Dong, Fang Zhao, Wei Cui, Li Li,* Fuping Lu*. Fast green FCF inhibits Aβ fibrillogenesis, disintegrates mature fibrils, reduces the cytotoxicity, and attenuates Aβ-induced cognitive impairment in mice, International Journal of Biological Macromolecules, 2021, 170: 33-41.

[8] Tao Li; Hongbin Wang; Jingwen Li; Luying Jiang; Hongwei Kang; Zehui Guo; Chen Wang; Wei Yang; Fufeng Liu*; Fuping Lu*; Yihan Liu*. Enzymatic characterization, molecular dynamics simulation, and application of a novel Bacillus licheniformis laccase, International Journal of Biological Macromolecules, 2021, 167: 1393-1405.

[9] 路福平, 黄爱岚, 赵蕾, 刘晓凤, 郭宵, 陈宁, 刘夫锋. 计算机模拟在食品工业用酶改造中的应用, 中国食品学报, 2020, 20(11): 1-10.

[10] Lin Huang, Jieying Ma, Jingcheng Sang, Nan Wang, Shuang Wang, Chen Wang, Hongwei Kang, Fufeng Liu*, Fuping Lu*, Yihan Liu*. Enhancing the thermostability of phospholipase D from Streptomyces halstedii by directed evolution and elucidating the mechanism of a key amino acid residue using molecular dynamics simulation, International Journal of Biological Macromolecules, 2020, 164: 3065-3074.

[11] Longgang Jia, Yuqing Liu, Wenliang Wang, Ying Wang, Haiqing Liu, Fufeng Liu, Rong Chen, Valina L. Dawson, Ted M. Dawson, Fuping Lu, Lei Liu*, Yanping Wang*, Xiaobo Mao*. Molecular mediating prion-like α-synuclein fibrillation from toxic PFFs to nontoxic species, ACS Applied Bio Materials, 2020, 3(9): 6096-6102.

[12] Xiao Guo, Yajing An, Chengcheng Chai, Jingcheng Sang, Luying Jiang, Fuping Lu, Yujie Dai, Fufeng Liu*. Construction of the R17L mutant of MtC1LPMO for improved lignocellulosic biomass conversion by rational point mutation and investigation of the mechanism by molecular dynamics simulations, Bioresource Technology, 2020, 317: 124024.

[13] Fufeng Liu#*, Wenping Zhao#, Fang Zhao, Qinchen Dong, Ying Wang, Wei Wei, Longgang Jia, Li Li, Fuping Lu*, Dual Effect of the Acidic Polysaccharose Ulvan on the Inhibition of Aβ Fibrillation and Disintegration of Mature Fibrils, ACS Applied Materials Interface, 2020, 12(37): 41167-41176. Top1区

[14] Xiao Guo, Jingcheng Sang, Chengcheng Chai, Yajing An, Zhifeng Wei, Yujie Dai, Fuping Lu*, Fufeng Liu*. A lytic polysaccharide monooxygenase from Myceliophthora thermophila C1 and its characterization in cleavage of glycosidic chain of cellulose. Biochemical Engineering Journal, 2020, 162: 107712.

[15] 郭宵，安亚静，柴成程，路福平，刘夫锋*。大肠杆菌分泌表达裂解性多糖单加氧酶发酵条件的优化, 食品与发酵工业, 2020, 46(5): 31-37.

[16] Jia, Longgang; Wang, Wenjuan; Yan, Yushan; Hu, Rui; Sang, Jingcheng; Zhao, Wenping; Wang, Ying; Wei, Wei; Cui, Wei; Yang, Guoqiang; Lu, Fuping*; Zheng, Jie*; Liu, Fufeng*. General Aggregation-Induced Emission Probes for Amyloid Inhibitors with Dual Inhibition Capacity against Amyloid beta-Protein and alpha-Synuclein, ACS Applied Materials Interface, 2020, 12 (28): 31182-31194.

[17] 郭宵，安亚静，柴成程，蒋露莹，路福平，刘夫锋٭，戴玉杰٭。来源于真菌的AA9家族裂解性多糖单加氧酶的研究进展，微生物学通报，2020, 47(7): 2309-2320.

[18] Longgang Jia, Wenping Zhao, Wei Wei, Xiao Guo, Wenjuan Wang, Ying Wang, Jingcheng Sang, Fuping Lu*, Fufeng Liu*. Expression and purification of amyloid β-protein, tau and α-synuclein in Escherichia coli: A review. Critical Reviews in Biotechnology, 2020, 40(4): 475-489.

[19] 位薇, 常保根, 王英, 路福平,* 刘夫锋*。Tau蛋白核心片段306~378的异源表达、纯化及聚集特性验证。中国生物工程杂志, 2020, 40(5): 22-29.

[20] Fufeng Liu*, Fang Zhao, Wenjuan Wang, Jingcheng Sang, Longgang Jia, Li Li, Fuping Lu*. Cyanidin-3-O-glucoside inhibits Aβ40 fibrillogenesis, disintegrates preformed fibrils, and reduces amyloid cytotoxicity. Food & Function, 2020, 11: 2573-2587.

[21] 贾龙刚，王英，路福平，刘夫锋*。α-突触核蛋白的高效表达纯化及表征。天津科技大学学报，2020, 35(2):1-5.

[22] Longgang Jia, Wenping Zhao, Jingcheng Sang, Wenjuan Wang, Wei Wei, Ying Wang, Fang Zhao, Fuping Lu*, Fufeng Liu*. Inhibitory effect of a flavonoid dihydromyricetin against Aβ40 amyloidogenesis and its associated cytotoxicity, ACS Chemical Neuroscience, 2019, 10: 4696-4703.

[23] Fufeng Liu*, Ying Wang, Jingcheng Sang, Wei Wei, Wenping Zhao, Beibei Chen, Fang Zhao, Longgang Jia, Fuping Lu*. Brazilin inhibits α-synuclein fibrillogenesis, disrupts mature fibrils and protects against amyloid-induced cytotoxicity, J Agric Food Chem, 2019, 67(42): 11769-11777. IF：3.571

[24] 赵文平，贾龙刚，路福平，刘夫锋*。基于β-内酰胺酶构建大肠杆菌体内Aβ42聚集抑制剂筛选体系, 食品与发酵工业, 2019, 45: 17-24.

[25] Yihan Liu, Lin Huang, Mengying Shan, Jingcheng Sang, Yanzhen Li, longgang Jia, Nan Wang, Shuang Wang, Shulin Shao, Fufeng Liu*, Fuping Lu*. Enhancing the activity and thermostability of Streptomyces mobaraensis transglutaminase by directed evolution and molecular dynamics simulation, Biochemical Engineering Journal, 2019, 151: 107333.  2区， IF:3.371

[26] Huitu Zhang&, Jingcheng Sang&, Ying Zhang&, Tongwei Sun, Huan Liu, Rong Yue, Jing Zhang, Haikuan Wang, Yujie Dai, Fuping Lu*, Fufeng Liu*. Rational design of a Yarrowia lipolytica derived lipase for improved thermostability, International Journal of Biological Macromolecules, 2019, 137: 1190-1198.

[27] Longgang Jia, Ying Wang, Wei Wei, Wenping Zhao, Fuping Lu*, Fufeng Liu*. Vitamin B12 inhibits α-synuclein fibrillogenesis and protects against amyloid-induced cytotoxicity. Food & Function, 2019, 10, 2861-2870.

[28] Longgang Jia#, Ying Wang#, Jingcheng Sang, Wei Cui, Wenping Zhao, Wei Wei, Beibei Chen, Fuping Lu*, Fufeng Liu*, Dihydromyricetin inhibits α-synuclein aggregation, disrupts the preformed fibrils and enhances cell viability, J Agric Food Chem, 2019, 67(14): 3946-3955.

[29] Qingmei Sun#, Fufeng Liu#, Jingcheng Sang, Miaoman Lin, Jiale Ma, Xiao Xiao, Sicheng Yan, C. Benjamin Naman, Ning Wang, Shan He, Xiaojun Yan, Wei Cui*, Hongze Liang*. 9-Methylfascaplysin is a more potent Aβ aggregation inhibitor than the marine-derived alkaloid, fascaplysin, and produces nanomolar neuroprotective effects in SH-SY5Y Cells, Marine drugs, 2019, 17(2): 121.

[30] 李丽，刘夫锋*。淀粉样β蛋白质构象转换及其抑制的分子动力学模拟，生物加工过程，2019,17（1）：44-52。

[31] Longgang Jia, Wenjuan Wang, Jingcheng Sang, Wei Wei, Wenping Zhao, Fuping Lu*, Fufeng Liu*. Amyloidogenicity and Cytotoxicity of a Recombinant C-terminal His6-tagged Aβ1-42. ACS Chemical Neuroscience. 2019, 10(3): 1251-1262.

[32] Fufeng Liu٭, Wenjuan Wang, Jingcheng Sang, Longgang Jia, Fuping Lu. Hydroxylated Single Wall Carbon Nanotube Inhibits Aβ1-42 Fibrillogenesis, Disaggregates Preformed Fibrils, and Protects against Aβ42-Induced Cytotoxicity. ACS Chemical Neuroscience. 2019, 10(1): 588-598.

[33] 刘夫锋, 李丽*。抗体亲和肽配基的高通量筛选和理性设计。天津科技大学学报，2019, 1: 1-11.

[34] 尚金召，王雪芹，熊聿力，路福平，刘夫锋*。hIAPP 与麦芽糖结合蛋白的融合表达、 纯化和鉴定。天津科技大学学报，2019, 34(6): 25-30.

[35] Shuhong Mao#; Jian-Wen Wang#; Fufeng Liu#; Zhangliang Zhu; Dengke Gao; Qianqian Guo; Panpan Xu; Zheng Ma; Yali Hou; Xiaotao Cheng; Dengyue Sun; Fuping Lu* and Hui-Min Qin*. Engineering of 3-ketosteroid-∆1-dehydrogenase based site-directed saturation mutagenesis for efficient biotransformation of steroidal substrates, Microbial Cell Factories, 2018, 17: 141.

[36] Yu Li, Xiaoyan Hu, Jingcheng Sang, Ying Zhang, Huitu Zhang, Fuping Lu٭, Fufeng Liu٭. An acid-stable β-glucosidase from Aspergillus aculeatus: Gene expression, biochemical characterization and molecular dynamics simulation, International Journal of Biological Macromolecules, 2018, 119: 462-469.

[37] Junying Wang, Ying Zhang, Xingji Wang, Jinzhao Shang, Yu Li, Huitu Zhang, Fuping Lu,٭ Fufeng Liu٭. Biochemical characterization and molecular mechanism of acid denaturation of a novel α-amylase from Aspergillus niger. Biochemical Engineering Journal, 2018, 137: 222-231.

[38] L. Jia, W. Wang, J. Shang, W. Zhao, W. Wei, Y. Wang, L. Li, F. Lu*, F.-F. Liu*. Highly Efficient Soluble Expression, Purification and Characterization of Recombinant Aβ42 from Escherichia coli, RSC Advances, 2018, 8: 18434-18441.

[39] N. Lu*, Y. Sun, Y. Ding, R. Tian, L. Li,* F.-F. Liu* Adsorption of human serum albumin on functionalized single-walled carbon nanotubes reduced cytotoxicity. Chemico-Biological Interactions, 2018, 295, 64-72.

[40] H. Qin, Z. Zhu, Z. Ma, P. Xu, Q. Guo, S. Li, J. Wang, S. Mao, F.-F. Liu*, and F.-P. Lu*. Rational design of cholesterol oxidase for efficient bioresolution of cholestane skeleton substrates, Scientific Reports, 2017, 7: 16375.

[41] J.-J. Guo#, W.-Q. Sun#, F.-F. Liu*. Brazilin inhibits the Zn2+-mediated aggregation of amyloid β-protein and alleviates cytotoxicity, Journal of Inorganic Biochemistry, 2017, 177: 183-189.

[42] J.-J. Guo#, W.-Q. Sun#, L. Li, F.-F. Liu*, W.-Y. Lu*. Brazilin Inhibits Fibrillogenesis of Human Islet Amyloid Polypeptide, Disassembles Mature Fibrils, and Alleviates Cytotoxicity, RSC Advances, 2017, 7: 43491-43501.

[43] 刘逸寒，王洪彬，刘夫锋，张会图，秦慧民，路福平. 微生物碱性蛋白酶的研究与开发，天津科技大学学报, 2017, 32(2): 1-6.

授权发明专利主要有：

[1] 刘夫锋，贾龙刚，赵文平，路福平，一种Aβ42聚集诱导发光融合体及其构建与应用，申请日：2019.08.01，申请号：2019107081695

[2] 刘夫锋，位薇，王英，贾龙刚，赵文平,张会图。一种具有阻抗蛋白聚集功能的Aβ42修饰蛋白及其表达与纯化方法，申请日：2019.06.28 申请号：2019105757453

[3] 刘夫锋，王英，赵文平，路福平。固绿在制备α-突触核蛋白聚集抑制剂中的用途，申请日：2019.07.24申请号：201910419084.5

[4] 刘夫锋，贾龙刚，路福平，王文娟，李玉，张会图。一种Aβ42修饰蛋白及其表达与纯化方法，申请日：2017.10.27，申请号：201711018671.0

[5] 刘夫锋，贾龙刚，路福平，王文娟，张会图，秦慧民，李玉。Aβ42在大肠杆菌中的高效可溶性表达及纯化方法，申请号：201710499265.4，申请日：2017.06.27，公开号：CN107245494A，公开日：2017.10.13

[6] 孙彦, 赵韦韦, 刘夫锋, 史清洪. 基于蛋白A亲和模型构建免疫球蛋白G的亲和配基多肽库及设计方法方法的应用. 专利号：ZL201210561815.8，授权公告日：2015.06.24

获奖情况

先后荣获香江学者、天津大学北洋学者青年骨干教师、天津大学大学生创新性实践计划项目优秀指导教师、天津大学本科毕业设计优秀指导教师、宝舜奖教金、天津大学第九届青年教师讲课大赛化工学院一等奖、天津大学第九届青年教师讲课大赛天津大学三等奖、Hong Kong Scholars Annual Symposium, Best poster、天津大学本科毕业设计优秀指导教师、第五届环渤海微生物学术研讨会优秀论文奖、中国化工学会年会优秀论文奖、“挑战杯”全国大学生系列科技学术竞赛天津科技大学银奖、第五届全国大学生生命科学创新创业大赛创业类三等奖、第五届全国大学生生命科学创新创业大赛优秀成果奖-指导教师三等奖等。

联系方式

办公地点：天津经济技术开发区第十三大街29号天津科技大学生物工程学院

邮政编码：300457

办公电话：022-60602717

传 真：022-60602298

Email：fufengliu@tust.edu.cn